Journal
JOURNAL OF NEUROSCIENCE METHODS
Volume 112, Issue 2, Pages 119-133Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0165-0270(01)00459-9
Keywords
dopamine; caudate-putamen; nucleus accumbens; voltammetry; release; uptake
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Funding
- NIDA NIH HHS [DA 08379] Funding Source: Medline
- NINDS NIH HHS [NS 15841, NS 35298] Funding Source: Medline
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Quantifying mechanisms underlying extracellular signaling by the neurotransmitter dopamine (DA) is a difficult task, particularly in the complex extracellular microenvironment of the intact brain. In this study, two methods for evaluating release and uptake from DA dynamics monitored by real-time voltammetry are described. Both are based on a neurochemical model characterizing electrically evoked levels of DA as a balance between these opposing mechanisms. The theoretical basis of what is called here nonlinear regression and single curve analyses is given. Fitting simulated data tests the reliability of the methods. The two analyses are also compared with an experimental data set describing the effects of pharmacologically inhibiting the DA transporter in the caudate-putamen (CP) and nucleus accumbens (NAc). The results indicate that nonlinear regression and single curve analyses are suitable for quantifying release and uptake mechanisms underlying DA neurotransmission. Additionally, the most important experimental finding of this technical study was the independent confirmation of high affinity ( approximate to 0.2 muM) DA uptake in the intact striatum. (C) 2001 Elsevier Science B.V. All rights reserved.
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