Journal
SYNAPSE
Volume 42, Issue 4, Pages 273-280Publisher
WILEY-LISS
DOI: 10.1002/syn.10012
Keywords
ketamine; dopamine transporter; [C-11]beta-CFT; [C-11]beta-CIT-FE; positron emission tomography; monkey brain
Categories
Ask authors/readers for more resources
The effects of ketamine anesthesia on the binding of [C-11]-labeled cocaine analogs, [C-11]beta -CFT (2 beta -carbomethoxy-3 beta-(4-fluorophenyl)tropane) and [C-11]beta -CIT-FE (N-(2-fluoroethyl)-2 beta -carbomethoxy-3 beta-(4-iodophenyl)tropane), to the striatal dopamine transporter (DAT) were evaluated in the monkey brain using positron emission tomography (PET). We sequentially measured the kinetics of these labeled compounds in the brains of five young-adult male rhesus monkeys (Macaca mulatta) in the conscious state, followed by those under ketamine anesthesia with continuous infusion (3 and 10 mg/kg/h). After intravenous injection, [C-11]beta -CFT and [C-11]beta -CIT-FE were predominantly accumulated in the striatum in both conscious and ketamine-anesthetized states. In the conscious state, the striatal uptake of [C-11]beta -CFT and [C-11]beta -CIT-FE continuously increased with time up to 91 min after injection. Continuous infusion of ketamine-induced higher levels of uptake of [C-11]beta -CFT and [C-11]beta -CIT-FE into the brain in a dose-dependent manner as compared with conscious state, and kinetic analysis with metabolite-corrected arterial input function indicated that the binding potentials (BP = k(3)/k(4)) of both compounds were elevated by ketamine. Not only [C-11]beta -CIT-FE but also [C-11]beta -CFT reached the equilibrium state of specific binding in the striatum within 40-50 min after injection. The present results demonstrated that ketamine significantly alters the DAT availability as measured with [C-11]beta -CFT and [C-11]beta -CIT-FE in the brain. (C) 2001 Wiley-Liss, Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available