Inductively coupled plasma mass spectrometry to identify protein drug targets from whole cell systems

Proteins from cisplatin treated bacterial cells were partially separated by ID polyacrylamide gel electrophoresis and analysed by laser ablation inductively coupled plasma mass spectrometry; using peptide fingerprinting methods central to proteomics, the band containing the highest levels of platinum was found to contain outer membrane protein A, which may be involved in cisplatin uptake.