Quantitative determination of sulfonamide in meat by liquid chromatography-electrospray-mass spectrometry

This paper describes a newly developed liquid chromatography-electrospray-mass spectrometry (LC-ES-MS) method for the quantitative determination of nine commonly used sulfonamides (sulfadiazine, sulfapyridine, sulfamerazine, sulfamethazine, sulfamonomethoxine, sulfisoxazole, sulfadimethoxine, sulfaquinoaline and sulfaphenazole) in meat. [M + H](+) and [M + Na](+) were the two major ions detected in positive ion mode. Selective ion monitoring was employed for quantitative determination. Satisfactory linearity, 0.1-10 mug ml(-1), of each compound was obtained. Blank meat samples were fortified at levels between 50 and 500 mug kg(-1). [Phenyl-(13)C6]sulfamethazine was used as internal standard. Sulfonamides were isolated from meat with a solvent extraction procedure and then determined by LC-ES-MS. The limits of detection were below 10 mug kg(-1). The application of this newly developed method was demonstrated by analyzing various beef, pork and chicken samples from local markets. (C) 2001 Elsevier Science B.V. All rights reserved.