Journal
FEBS LETTERS
Volume 510, Issue 1-2, Pages 27-30Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(01)03219-7
Keywords
bulge-helix-bulge motif; pre-mRNA splicing; Cbf5p; Aeropyrum pernix; Sulfolobus solfataricus; Sulfolobus tokodaii
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Introns in protein-coding genes are ubiquitous in eukaryotic cells, but pre-mRNA splicing has yet to be reported in archaeal and its viral genomes. We present evidence of introns in genes encoding a homolog of eukaryotic Cbf5p (centromere-binding factor 5; a subunit of a small nucleolar ribonucleoprotein) in three Archaea; Aevopyrum pernix, Sulfolobus solfataricus and Sulfolobus tokodaii. Splicing of pre-mRNAs in vivo was demonstrated by reverse transcriptase-mediated polymerase chain reaction. The exon-intron boundaries of these genes are predicted to be folded into a structure similar to the bulge-helix-bulge motif, suggesting that splicing of these pre-mRNAs probably depends on the splicing system elucidated for archaeal pre-tRNAs and rRNAs. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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