Journal
FEBS LETTERS
Volume 510, Issue 1-2, Pages 77-82Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(01)03220-3
Keywords
nuclear receptor; progesterone receptor beta; progestogen; spermiation; sperm maturation; Japanese eel
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A cDNA encoding a second type of a progestogen receptor (ePR2) was isolated from the same library as we had previously cloned a functional PR (ePR1) in eel testis. The amino acid sequence of the ePR2 shows low homology with ePR1 (34%), but both PRs showed progestogen-dependent transactivation in transfection experiment. Tissue distribution of ePR2 mRNA was clearly different from that of ePR1. Protein interaction between two PRs was demonstrated in vitro by a glutathione S-transferase pull-down assay. These results indicate that ePR2 is also a functional PR. This is the first isolation of two different functional PR molecules from a vertebrate. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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