Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 766, Issue 1, Pages 115-121Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(01)00460-1
Keywords
carbon monoxide
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In order to measure changes in physiological CO concentrations in blood with good accuracy, a method was developed using gas chromatography with flame ionisation detection (250degreesC). A nickel catalyst system was fitted to convert CO to methane at 375degreesC after separation with a molecular sieve column at 35degreesC. Helium was used as carrier at 30 ml/min. Porcine or human blood (400 p,1) was sampled in gastight tubes and treated with sulfuric acid and saponin (800 mul). Accuracy was 1.4% and 1.5% (RSD), respectively. Precision was 2.8% (porcine blood). Limit of detection was 0.01 nmol/ml gas and limit of quantification 12 nmol/ml blood. Calibration was made in the interval 12-514 nmol/ml blood (corresponding to 0.1-6% COHb). Samples were stable for at least a month at +4degreesC. This paper describes a method with high sensitivity and good accuracy, suitable for analysis of low CO concentrations. (C) 2002 Elsevier Science B.V. All rights reserved.
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