Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 77, Issue 2, Pages 219-224Publisher
WILEY
DOI: 10.1002/bit.10131
Keywords
human secreted alkaline phosphatase (SEAP); baculovirus expression vector system (BEVS); insect cells; protein glycosylation; dissolved oxygen (DO) concentration
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The effect of dissolved oxygen concentration on human secreted alkaline phosphatase (SEAP) glycosylation by the insect cell-baculovirus expression system was investigated in a well-controlled bioreactor. Oligomannose-type N-linked glycans (i.e., Man2 to Man6 and Man3F) were present in SEAP produced by Spodoptera frusiperda Sf-9 (Sf-9) and Trichoplusia ni BTI-Tn-5B1-4 (Tn-5B1-4) insect cell lines. The relative amounts of the most highly processed glycans (i.e., Man3F and Man2 in the SEAP from Sf-9 and Tn-5B1-4 cells, respectively) were significantly higher at 50% of air saturation than at either 10% or 190% of air saturation. That is, glycan processing was inhibited at both low and high dissolved oxygen concentrations. (C) 2002 John Wiley Sons, Inc.
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