Journal
JOURNAL OF EXPERIMENTAL MEDICINE
Volume 195, Issue 2, Pages 259-268Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20010838
Keywords
antigen presentation; dendritic cells; lymph node; MHC antigens; transplantation
Categories
Funding
- NEI NIH HHS [EY12963, R01 EY012963, R01 EY010752, EY10752, R29 EY010752] Funding Source: Medline
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To examine the widely accepted dogmas that corneal grafts lack passenger leukocytes or cells capable of migrating directly to lymph nodes (LNs), we tracked the migration of corneal graft-derived transgenic green fluorescent protein (GFP; Ia(b)) cells into the draining LNs of allogeneic (lad) recipients. GFP(+) cells were identified in cervical LNs several hours after transplantation, and this traffic was significantly enhanced when grafts were placed in inflamed recipient beds. Draining cells were la(b+), CD45(+), and CD11c(+), and examination of ungrafted corneas revealed numerous similarly CD45(+)CD11c(+)CD3(-)CD8alpha(-) cells that uniformly lacked major histocompatibility complex (MHC) class II expression; transmission electron microscopy confirmed the presence of morphologically similar cells. After transplantation, or placement in culture, these CD11c(+) cells became class II+ in a time-dependent manner and were capable of allostimulatory function. However, the stimulatory capacity of these cornea-derived dendritic cells (DCs) was suppressed compared with splenic controls. These results demonstrate for the first time that the cornea is endowed with resident DCs that are universally MHC class II- but that are capable of expressing class 11 antigen after surgery and migrating to draining LNs of allografted hosts. These data refute the tenet that the cornea is immune privileged due to lack of resident lymphoreticular cells or due to antigenic sequestration front systemic immunity.
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