Journal
BMC CELL BIOLOGY
Volume 3, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/1471-2121-3-3
Keywords
lectin; galectin-1; site-directed mutagenesis; cell proliferation; growth control
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Background Previous work, by us and others, has shown that mammalian galectins-1 have a growth-inhibitory activity for mammalian cells which is apparently independent of their beta-galactoside binding site. Results We have made recombinant human galectin-1 as a bacterial fusion protein with an N-terminal hexahistidine tag. This protein displays both haemagglutination and growth-inhibitory activities, even in the presence of the hexahistidine tag. Site-directed mutagenesis of this protein has confirmed the independent nature of the protein sites responsible for the two biological activities. Mutant proteins were created, which displayed each activity in the absence of the other. Conclusions Human galectin-1 possesses a growth-inhibitory site, which is not part of the beta-galactoside binding site. A surface loop, comprising amino acid residues 25-30, and joining two internal beta-strands, forms part of the growth-inhibitory site. This region is relatively close to the N-terminus of the protein, and N-terminal substitutions of extensions also affect growth-inhibitory activity. Further experiments will be necessary to fully define this site.
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