Journal
JOURNAL OF VIROLOGY
Volume 76, Issue 4, Pages 2003-2008Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.76.4.2003-2008.2002
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Funding
- NIAID NIH HHS [R21 AI043513] Funding Source: Medline
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Astrovirus contains three open reading frames (ORF) on its genomic RNA, ORF1a, ORF1b, and ORF2. ORF1a encodes a 920-amino-acid (aa) nonstructural protein, nsP1a, which displays a 3C-like serine protease motif. Little is known about the processing of nsP1a or whether the protease it contains is active and involved in autocatalytic processing. Here we address both of these matters. Intact and N-terminally deleted forms of ORF1a from human astrovirus serotype 1 were expressed in BHK cells, and nsP1a-derived processing products were immunoprecipitated with an nsP1a-specific antibody or an antibody specific for an N-terminally linked epitope tag. The mapping of the main processing products, p20 and p27, suggests cleavage sites near as 170, 410, and 655 of nsP1a. Cleavages at around as 410 and 655, but not as 170, were abolished when a 9-aa substitution was introduced into the protease motif in nsP1a. The p27 processing product was also found in Caco-2 cells that had been infected with human astrovirus serotype 1, confirming the presence of the cleavage sites at approximately as 410 and 655.
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