Journal
AMERICAN JOURNAL OF BOTANY
Volume 89, Issue 2, Pages 279-286Publisher
WILEY
DOI: 10.3732/ajb.89.2.279
Keywords
AFLP; in situ hybridization; ITS; FISH; GISH; Miscanthus; Poaceae; polyploidy; trnL-F
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Two clones of Miscanthus, grown under the names M. Xgiganteus and M. sacchariflorus, have been used in biomass trials in Europe, but neither the identity of these clones nor their origin has been established DNA sequencing, amplified fragment length polymorphism (AFLP), and chromosome studies confirm that M. Xgiganteus. is an allotriploid (2n = 3x = 57) combining genomes from M sinensis (2n = 2x = 38) and M. sacchariflorus (2n = 38 or 76). Two alleles of the internal transcribed spacer of 18S-25S nuclear ribosomal DNA (ITS) were discovered in polymerase chain reaction products of M. Xgiganteus. Cloning of these revealed that one matched M. sinensis and the other M. sacchariflorus. Plastid trnL, intron and trnL-F spacer sequences showed that the maternal lineage of M. Xgiganteus was M. sacchariflorus. Fluorescent in situ hybridization, FISH, was used to investigate genome organization in Miscanthus but was unable to differentiate between the different parental genomes present in M. Xgiganteus, indicating that two parental genomes are still extremely similar at the repetitive DNA level This study is an example in which rDNA sequences and AFLP fingerprints permit identification of the parental genomes in a hybrid, but FISH methods, at the repetitive DNA level (including genomic in situ hybridization, GISH), were unable to do so because their sequences remain too similar.
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