Journal
BIOLOGY OF THE CELL
Volume 94, Issue 1, Pages 15-27Publisher
WILEY
DOI: 10.1016/S0248-4900(01)01178-9
Keywords
Caco-2 cell; intestinal differentiation; peroxisome; peroxisome proliferator-activated receptor
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The expression of peroxisome proliferator-activated receptors alpha (PPARalpha) and gamma (PPARgamma) was studied in the human adenocarcinoma Caco-2 cells induced to differentiate by long term culture (15 days). The differentiation of Caco-2 cells was attested by increases in the activities of sucrase-isomaltase and alkaline phosphatase (two brush border enzymes), fatty acyl-CoA oxidase (AOX) and catalase (two peroxisomal enzymes), by an elevation in the protein levels of villin (a brush border molecular marker), AOX, peroxisomal bifunctional enzyme (PBE), catalase and peroxisomal membrane protein of 70 kDa (PMP70), and by the appearance of peroxisomes. The expression of PPARalpha and PPARgamma was investigated by Western blotting, immunocytochemistry, Not-them blotting and S1 nuclease protection assay during the differentiation of Caco-2 cells. The protein levels of PPARalpha, PPARgamma, and PPARgamma(2) increased gradually during the time-course of Caco-2 cell differentiation. Immunocytochemistry revealed that PPARalpha and gamma were localized in cell nuclei. The PPARgamma(1) protein was encoded by PPARgamma(3) mRNA because no signal was obtained for PPARgamma(1) mRNA using a specific probe in S I nuclease protection assay. The amount of PPARgamma(3) mRNA increased concomitantly to the resulting PPARgamma(1) protein. On the other hand, the mRNA of PPARalpha and PPARgamma(2) were not significantly changed, suggesting that the increase in their respective protein was due to an elevation of the translational rate. The role played by the PPAR subtypes in Caco-2 cell differentiation is discussed. (C) 2002 Editions scientifiques et medicales Elsevier SAS.
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