Journal
EUROPEAN JOURNAL OF BIOCHEMISTRY
Volume 269, Issue 3, Pages 915-922Publisher
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.0014-2956.2001.02727.x
Keywords
Saccharomyces cerevisiae; glyoxylate cycle; peroxisome; malate synthase 1; oleic acid
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The eukaryotic glyoxylate cycle has been previously hypothesized to occur in the peroxisomal compartment. which in the yeast Saccharomyces cerevisiae additionally represents the sole site for fatty acid beta-oxidation. The subcellular location of the key glyoxylate-cycle enzyme malate synthase 1 (Mls1p), an SKL-terminated protein. was examined in yeast cells grown on different carbon sources. Immunoelectron microscopy in combination with cell fractionation showed that Mls1p was abundant in the peroxisomes of cells grown on oleic acid. whereas in ethanol-grown cells Mls1p was primarily cytosolic. This was reinforced using a green fluorescent protein (GFP) Mls1p reporter, which entered peroxisomes solely in cells grown under oleic acid-medium conditions. Although growth of cells devoid of Mls1p on ethanol or acetate could be fully restored using a cytosolic Mls1p devoid of SKL. this construct could only partially alleviate the requirement for native Mls1p in cells grown on oleic acid. The combined results indicated that Mls1p remained in the cytosol of cells grown on ethanol. and that targeting of Mls1p to the peroxisomes was advantageous to cells grown on oleic acid as a sole carbon source.
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