Effects of various elicitors on the transcription of a β-1,3-endoglucanase gene in citrus fruit

Very little is yet known regarding the molecular mechanisms involved in pathogen defense responses in citrus fruit. Recently, a basic beta-1,3-endoglucanase (EC 3.2,2.39) belonging to the pathogenesis-related (PR) group of proteins, has been purified from Citrus sinensis (L) Osbeck cv. 'Valencia' orange callus. Specific antibodies raised against the purified protein were used to screen 'Valencia' callus and flavedo cDNA expression libraries. and to isolate its corresponding cDNA, designated gnsl. The gnsl gene encodes a predicted polypeptide of 336 amino acids with a molecular mass of 37.3 kDa and a basic pI of 9.19. and shares 55-65% identity with several other plant beta-1,3-endoglucanase proteins. Hereby, we show that the expression of the gnsl gene is markedly induced by wounding and inoculation with Penicillium digitatum (Pers. Fr.) Sacc., and following treatments with various elicitors that induce fruit resistance against P. digitatum. These treatments include UV irradiation. application of jasmonic acid (JA), beta-aminobutyric acid (BABA), Candida oleophila antagonist yeast cells and hot water rinsing and brushing. Overall. based on various RNA gel blot hybridizations. we assume that gnsl is most likely to be part of the molecular mechanisms involved in pathogen defense responses in citrus fruit.