Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 48, Issue 2-3, Pages 149-160Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0167-7012(01)00319-0
Keywords
HPLC-ESI-MS; marine sediments; microbial community structure; phospholipids; sulfate-reducing bacteria
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To complement information on microbial communities in marine sediments that can be obtained using microbiological methods, we developed an analytical procedure to trace microbial lipids in environmental samples. We focused on analyzing intact phospholipids as these membrane constituents are known to be biomarkers for viable cells. Analysis of intact phospholipids From a fractionated and preconcentrated sediment extract was achieved using liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). The combined analysis of phospholipid types and their fatty acid substituents allowed a differentiation between various groups of microorganisms living in the sediment. For comparison three strains of marine sulfate-reducing bacteria (SRB) were analysed for their lipid content. (C) 2002 Elsevier Science B.V. All rights reserved.
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