Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 48, Issue 2-3, Pages 101-106Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0167-7012(01)00314-1
Keywords
matrix-assisted laser desorption/ionization; 16S rDNA; time-of-flight mass spectrometry
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Recently, we demonstrated that a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) can be used to determine the molecular weight of polymerase chain reaction (PCR) products of intact 16S rRNIA regions and to profile their restriction digests. This is the first time that MALDI-TOF MS with ultraviolet (UV) photoionization has been used to analyze a PCR product of similar to 1600 nucleotides in length. (C) 2002 Elsevier Science B.V. All rights reserved.
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