Journal
ZYGOTE
Volume 10, Issue 1, Pages 73-78Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0967199402002101
Keywords
antral oocytes; chromatin organisation; developmental competence; preimplantation development; mouse
Funding
- Telethon [A.132] Funding Source: Medline
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Mouse antral oocytes can be classified in two different types termed SN or NSN oocytes, depending on the presence or absence, respectively, of a ring of Hoechst 33342-positive chromatin surrounding the nucleolus. The aim of the present study was to test the developmental competence to blastocyst of the two types of oocytes. Here we show that following isolation, classification and culture of cumulus-free antral oocytes, 14.7% and 74.5% of NSN and SN oocytes, respectively, reached the metaphase 11 stage. When fertilised and further cultured none of the metaphase 11 NSN oocytes developed beyond the 2-cell stage whilst 47.4%. of the metaphase 11 SN oocytes reached the 4-cell stage and 18.4% developed to blastocyst, The findings reported in this paper may contribute to improved procedures of female gamete selection for in vitro fertilisation of humans and farm animals. Furthermore, the selection of oocytes with better developmental potential may be of interest for studies on nuclear/cytoplasm interaction, particularly in nuclear-transfer experiments.
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