Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 99, Issue 3, Pages 1158-1163Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.032658999
Keywords
biosynthesis of isoprenoids; 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate isopentenyl diphosphate; dimethylallyl diphosphate
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Isopentenyl diphosphate and dimethylallyl diphosphate serve as the universal precursors for the biosynthesis of terpenes. Although their biosynthesis by means of mevalonate has been studied in detail, a second biosynthetic pathway for their formation by means of 1-deoxy-D-xylulose 5-phosphate has been discovered only recently in plants and certain eubacteria. Earlier in vivo experiments with recombinant Escherichia coli strains showed that exogenous 1-deoxy-D-xylulose can be converted into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate by the consecutive action of enzymes specified by the xylB and ispCDEFG genes. This article describes the transformation of exogenous [U-C-13(5)]1-deoxy-D-xylulose into a 5:1 mixture of [U-C-13(5)]isopentenyl diphosphate and [U-C-13(5)]dimethylallyl diphosphate by an E. coli strain engineered for the expression of the ispH (lytB) gene in addition to recombinant xylB and ispCDEFG genes.
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