Fractionation of cytochromes of phototrophically grown Chloroflexus aurantiacus.: Is there a cytochrome bc complex among them?

The cytochrome-containing membrane complexes of the phototrophically grown green non-sulfur bacterium Chloroflexus aurantiacus were fractionated by anion exchange chromatography. Three cytochrome h and four cytochrome c peaks were observed. None of the separated complexes met the features of the cytochrome bc complex. Two main cytochrome b-containing complexes were further purified: a dimer of identical subunits with unknown function and a succinate:quinone oxidoreductase containing three subunit species. Two novel multisubunit complexes, similar to each other, with two heme c-bearing subunits were also purified. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.