Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 99, Issue 4, Pages 1910-1914Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.032594399
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Funding
- NCI NIH HHS [CA80882, R01 CA080882] Funding Source: Medline
- NIGMS NIH HHS [GM19261] Funding Source: Medline
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Both in yeast and humans, DNA polymerase (Pol) eta functions in the error-free replication of UV-damaged DNA, and Poleta has the unique ability to efficiently replicate through a cis-syn thymine-thymine (T-T) dimer by inserting two As opposite the two Ts of the dimer. Although human DINB1-encoded Polkappa belongs to the same protein family as Poleta, Polkappa shows no ability to bypass this DNA lesion and its biological function has remained unclear. Here, we examine Polkappa for its ability to extend from primer-terminal mispairs opposite nondamaged and damaged DNA templates. We find that Polkappa is a promiscuous extender of primer-terminal mispairs opposite nondamaged DNA templates, and interestingly, it is also very efficient at extending from a G opposite the 3'T of a T-T dimer. These observations provide biochemical evidence for a role Of Polkappa in the extension of mismatched base pairs during normal DNA replication, and in addition, they implicate Polkappa in the mutagenic bypass of T-T dimers. In its proficient mismatch extension ability, Polkappa is more similar to the unrelated DNA polymerase than it is to the phylogenetically related Poleta or Poliota. Thus, in humans, Polkappa would compete with Polzeta for the extension of mismatched base pairs on damaged and undamaged DNAs.
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