Journal
JOURNAL OF CELL BIOLOGY
Volume 156, Issue 5, Pages 775-781Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200109018
Keywords
HSF1 granules; heterochromatin; nucleus; satellite III; stress
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Funding
- NIGMS NIH HHS [R37 GM038109, R01 GM038109, GM38109] Funding Source: Medline
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Activation of the mammalian heat shock transcription factor (HSF)1 by stress is a multistep process resulting in the transcription of heat shock genes. Coincident with these events is the rapid and reversible redistribution of HSF1 to discrete nuclear structures termed HSF1 granules, whose function is still unknown. Key features are that the number of granules correlates with cell ploidy, suggesting the existence of a chromosomal target. Here we show that in humans, HSF1 granules localize to the 9q11-q12 heterochromatic region. Within this locus, HSF1 binds through direct DNA-protein interaction with a nucleosome-containing subclass of satellite III repeats. HSF1 granule formation only requires the DNA binding competence and the trimerization of the factor. This is the first example of a transcriptional activator that accumulates transiently and reversibly on a chromosome-specific heterochromatic locus.
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