Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
Volume 59, Issue 3, Pages 499-506Publisher
JOHN WILEY & SONS INC
DOI: 10.1002/jbm.10015
Keywords
current-conducting biomaterials; osteoblast functions; electrical stimulation; carbon nanotubes
Funding
- NHLBI NIH HHS [HL60359] Funding Source: Medline
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The present study demonstrates that novel nanocomposites consisting of blends of polylactic acid and carbon nanotubes effectively can be used to expose cells to electrical stimulation. When osteoblasts cultured on the surfaces of these nanocomposites were exposed to electric stimulation (10 muA at 10 Hz) for 6 h/day for various periods of time, there was a 46% increase in cell proliferation after 2 days, a 307% increase in the concentration of extracellular calcium after 21 consecutive days, and upregulation of mRNA expression for collagen type-I after both 1 and 21 consecutive days. These results provide evidence that electrical stimulation delivered through novel, current-conducting polymer/nanophase composites promotes osteoblast functions that are responsible for the chemical composition of the organic and inorganic phases of bone. Furthermore, this evidence elucidates aspects of the cellular/molecular-level mechanisms involved in new bone formation under electrical stimulation. (C) 2001 John Wiley & Sons, Inc. J Biomed Mater Res 59: 499-506, 2002.
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