Journal
SCIENCE
Volume 295, Issue 5561, Pages 1910-1912Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1065028
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- NCI NIH HHS [CA83229] Funding Source: Medline
- NHGRI NIH HHS [HG00057] Funding Source: Medline
- NIGMS NIH HHS [GM062144] Funding Source: Medline
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Time courses of translocation of fluorescently conjugated proteins to the plasma membrane were simultaneously measured in thousands of individual rat basophilic leukemia cells. We found that the C2 domain-a calcium-sensing, lipid-binding protein module that is an essential regulator of protein kinase C and numerous other proteins-targeted proteins to the plasma membrane transiently if calcium was released from internal stores, and persistently in response to entry of extracellular calcium across the plasma membrane. The C2 domain translocation time courses of stimulated cells clustered into only two primary modes. Hence, the reversible recruitment of families of signaling proteins from one cellular compartment to another is a rapid bifurcation mechanism for inducing discrete states of cellular signaling net works.
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