Journal
AUSTRALIAN JOURNAL OF CHEMISTRY
Volume 62, Issue 10, Pages 1344-1350Publisher
CSIRO PUBLISHING
DOI: 10.1071/CH09208
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Funding
- Australian Research Council (ARC)
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Block copolymers of allyl methacrylate and N-(2-hydroxypropyl) methacrylamide (HPMA) with different block lengths have been synthesized by reversible addition-fragmentation chain transfer polymerization. Allyl groups were modified with cysteamine, via a thiol-ene photoreaction, with a high efficiency (similar to 100%)as evidenced by NMR spectroscopy, yielding cationic copolymers of HPMA. Polyelectrolyte complexes of small interfering RNAs (siRNA) and the cationic block copolymers were then formed at an N/P ratio between 1 and 4 depending on the block length of the copolymers. Increasing the length of the hydrophilic block was found to decrease the efficiency of siRNA complexation. The hydrodynamic diameter of the polyplexes in 130 mM buffer solution was less than 100 nm.
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