Journal
JOURNAL OF BIOTECHNOLOGY
Volume 94, Issue 1, Pages 125-132Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0168-1656(01)00423-0
Keywords
bioluminescence; Escherichia coli; fabA; fatty acid biosynthesis; microbial biosensors; toxicity bioassay
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A set of genetically engineered Escherichia coli strains was constructed, in which the promoter of the fabA gene is fused to Vibrio fischeri luxCDABE either in a multi-copy plasmid or as a single copy chromosomal integration. The fabA gene codes for beta-hydroxydecanoyl-ACP dehydrase, a key enzyme in the synthesis of unsaturated fatty acids, and is induced when fatty acid biosynthesis pathways are interrupted. A dose-dependent and highly sensitive bioluminescent response to a variety of chemicals was controlled by the fadR gene. A tolC mutant E. coli host displayed generally lower detection threshold for toxicants. A chromosomal integration of a single copy of the fabA'::lux fusion led to a markedly lower background luminescence, but did not yield an improvement in overall performance. It is proposed that these or similarly constructed reporters of fatty acid biosynthesis inhibition may serve as novel microbial toxicity biosensors. (C) 2002 Elsevier Science B.V. All rights reserved.
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