Journal
FEMS MICROBIOLOGY LETTERS
Volume 209, Issue 1, Pages 53-56Publisher
ELSEVIER SCIENCE BV
DOI: 10.1111/j.1574-6968.2002.tb11108.x
Keywords
preparation of N-acetylchitooligosaccharide; carbon source; intestinal bacterium; maximum cell density; specific growth rate
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N-Acetylehitooligosaccharides ((GlcNAc)(n)) with different degrees of polymerization (n=1-6) were prepared as the main carbon sources in media for evaluating the growth of nine intestinal bacteria. A chitohydrolysate was prepared by hydrolyzing shrimp-shell chitin using HCl. After purification, the purity of each (GlcNAc)(1-6) was > 86%. The growth of intestinal bacteria was carried out in a basal medium (BM) containing 0.2% (w/v) of each sugar or glucose as the main carbon source and was evaluated using maximum cell densities and specific growth rates. Bacteroides fragilis and Clostridium perfringens could respectively utilize GlcNAc and (GlcNAc)(2) more efficiently for growth than glucose. Bifidobacterium adolcscentis and Eubacterium limosum could use (GlcNAc)(1-6) slightly as their main carbon source. Escherichia coli, Lactococcus lactis and Proteus vulgaris could utilize glucose more efficiently than (GleNac)(1-6). GlcNAc was used more readily than (GlcNAC)(2-6) by Staphylococcus aureus, exhibiting almost the same specific growth rates. In BM, Streptococcus faecalis grew well even without adding each of the sugars tested. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.
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