Journal
YEAST
Volume 19, Issue 5, Pages 449-457Publisher
WILEY
DOI: 10.1002/yea.845
Keywords
Saccharomyces cerevisiae; aspartate carbamoyltransferase; dihydro-orotate dehydrogenase; computation analysis; hydrophobic helices; subcellular fractionation; membranes
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Computational analysis predicted three potential hydrophobic transmembrane a-helices within the Ura2 multidomain protein of Saccharomyces cerevisiae, the C-terminal subdomain of which catalyses the second step of uridine-monophosphate biosynthesis by its L-aspartate carbamoyltransferase activity (EC 2.1.3.2). The fourth step of pyrimidine biosynthesis is catalysed by dihydro-orotate dehydrogenase (Ura1 protein; EC 1.3.99.11), which was similarly characterized as a peripheral membrane protein. Ex situ, the activities of the investigated enzymes were associated both with isolated yeast membranes, fractionated by differential centrifugation to remove intact nuclei, and with soluble cytoplasmic proteins. Copyright (C) 2002 John Wiley Sons, Ltd.
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