3.8 Article Proceedings Paper

Analysis of the temporal expression of nestin in human fetal brain derived neuronal and glial progenitor cells

Journal

DEVELOPMENTAL BRAIN RESEARCH
Volume 134, Issue 1-2, Pages 87-92

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/S0165-3806(01)00325-X

Keywords

stem cell; glia; astrocyte; astrocyte progenitor; neuronal progenitor; GFAP

Ask authors/readers for more resources

Nestin expression in the developing human brain was examined with the use of unique human specific anti-nestin antibodies. Double immunostaining of cell cultures and tissue sections derived from first and second trimester human fetal brain (HFB) examined the co-expression of nestin with other cell type specific phenotypic markers. The immunocytochemical analysis shows that from first to second trimester, the majority of developing glial cells exhibited a transitional state marked by cc-expression of nestin and GFAP. However, the corresponding transitional state for developing neuronal cells, co-expressing nestin and MAP-2, was rarely detected. These results imply different temporal patterns of nestin expression in cells of glial and neuronal lineages. Confocal microscopy of HFB tissue section staining also revealed a similar pattern of nestin co-expression with glial and neuronal markers. Our results suggest that nestin expression alone may not identify an undifferentiated stein cell, and that progenitor cells in glial and neuronal lineages express nestin in different temporal patterns. Published by Elsevier Science B.V.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

3.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available