Journal
ANIMAL GENETICS
Volume 33, Issue 2, Pages 140-144Publisher
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1365-2052.2002.00817.x
Keywords
CA repeat; linkage mapping; marker generation; microsatellite
Ask authors/readers for more resources
We have developed (CA), microsatellite markers for the cichlid fish. Oreochromis niloticus using a variation of the hybrid capture method. The resulting genomic library was highly enriched in repetitive DNA with 96%, of clones containing CA repeats. The number of repeats ranged from four to 45 with an average of 19. Two-thirds of the sequenced clones had 12 or more repeats and sufficient flanking sequence to design primers. The resulting markers were tested in an F, cross of O. niloticus x O. aureus. Nearly 90% of the markers amplified in this cross and 74% of these were informative. This work demonstrates the importance of minimizing the number of polymerase chain reaction (PCR) amplification cycles before and after the enrichment steps to reduce PCR recombination and the generation of chimaeric clones.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available