Journal
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
Volume 77, Issue 4, Pages 495-499Publisher
JOHN WILEY & SONS LTD
DOI: 10.1002/jctb.595
Keywords
aqueous two-phase systems; chicken egg white proteins; partition; polyelectrolyte; polyethyleneoxidemaleic acid copolymer
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Aqueous two-phase systems were formed from solutions of a polyelectrolyte, polyethyleneoxide-maleic acid copolymer, and potassium phosphate. The properties of such aqueous two-phase systems were highly dependent on pH. This was reflected in the partition behavior of three chicken egg white proteins: lysozyme, conalbumin and ovalbumin. Separability of these three proteins was improved by the use of the polyelectrolyte, in comparison with when uncharged polyethylene glycol (poly(1,2-dihydroxyethane)) was used as a phase-forming polymer. (C) 2002 Society of Chemical Industry.
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