Journal
TALANTA
Volume 56, Issue 5, Pages 949-957Publisher
ELSEVIER
DOI: 10.1016/S0039-9140(01)00655-5
Keywords
DNA biosensor; toxicity; screen-printed electrodes; Toxalert (R) 100
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Applications of a disposable electrochemical DNA biosensor to standard solutions and to real samples are reported. The DNA biosensor is assembled by immobilising the double stranded calf thymus DNA on the surface of a disposable carbon screen-printed electrode. The immobilised ds-DNA interacts with the sample for 2 min; then is washed and immersed in a clean buffer where the analytical signal (the oxidation peak area of the guanine base) is obtained by a square-wave voltammetric scan. The results were compared with some currently used toxicity tests and in particular with a commercial luminescent bacteria test, Toxalert(R)100. (C) 2002 Elsevier Science B.V. All rights reserved.
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