Journal
NATURE STRUCTURAL BIOLOGY
Volume 9, Issue 4, Pages 278-281Publisher
NATURE AMERICA INC
DOI: 10.1038/nsb778
Keywords
-
Ask authors/readers for more resources
A synaptic complex of Tn 5 transposase with an extended outside end DNA duplex was prepared and crystallized, and its crystal structure was determined in an effort to reveal the role of metal ions in catalysis. Two Mn2+ ions bound to the active site when a single nucleotide of donor DNA was added to the 3' end of the transferred strand. Marked conformational changes were observed in the DNA bases closest to the active site. The position of the metal ions and the conformational changes of the DNA provide insight into the mechanism of hairpin formation and cleavage, and is consistent with a two-metal model for catalysis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available