Journal
MOLECULAR GENETICS AND GENOMICS
Volume 267, Issue 2, Pages 186-201Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00438-002-0650-0
Keywords
genetic diversity; long-primer PCR (LP-PCR); restriction fragment length polymorphisin (RFLP); simple sequence repeats (SSRs); stress responsive genes (SRG)
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Three types of molecular markers have been compared for their utility in evaluating genetic diversity among cultivars of Hordeum vulgare. Restriction fragment length polymorphisms at 71 sites were scored with the aid of probes corresponding to stress-responsive genes from barley and wheat, coding for a low-molecular weight heat shock protein, a dehydrin, an aldose reductase homolog, and a 18.9-kDa drought-induced protein of unknown function. Indexes of genetic diversity computed in the total sample and within groups of cultivars (two-rowed and six-rowed, winter and spring varieties) indicated high values of genetic differentiation (F-ST > 15%). A second assessment of genetic diversity was performed by PCR amplification of genomic DNA using as primers 13 arbitrary oligonucleotides derived from sequences of the same stress-responsive genes. A high degree of polymorphism was uncovered using these markers also, but they yielded low values for FST (< 7%) among groups of cultivars. Finally, 15 different simple-sequence repeats (AC or AG) were amplified with primers based on unique flanking sequences. Levels of polymorphism and differentiation between groups of cultivars revealed by these markers were quite high. Ordination techniques applied to measures of genetic distance among cultivars demonstrated a remarkable ability of the RFLPs associated with stress-responsive genes to discriminate on the basis of growth habit. The correlation with production data for the cultivars in different environments was also significant. This functional genomics strategy was therefore as informative as the structural genomics (SSR-based) approach, but requires the analysis of fewer probes.
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