Journal
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
Volume 282, Issue 4, Pages R969-R978Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpregu.00405.2001
Keywords
microvasculature; adenosine; nitric oxide; adenosine 5 '-triphosphate-sensitive potassium channels; metabolic control of blood flow
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Funding
- NHLBI NIH HHS [R01-HL-56574] Funding Source: Medline
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To determine whether nitric oxide (NO), adenosine (Ado) receptors, or ATP-sensitive potassium (K-ATP) channels play a role in arteriolar dilations induced by muscle contraction, we used a cremaster preparation in anesthetesized hamsters in which we stimulated four to five muscle fibers lying perpendicular to a transverse arteriole (maximal diameter similar to35-65 mum). The diameter of the arteriole at the site of overlap of the stimulated muscle fibers (the local site) and at a remote site similar to1,000 mum upstream (the upstream site) was measured before, during, and after muscle contraction. Two minutes of 4-Hz muscle stimulation (5-15 V, 0.4 ms) produced local and upstream dilations of 19 +/- 1 and 10 +/- 1 mum, respectively. N-omega-nitro-L-arginine (10(-4) M; NO synthase inhibitor), xanthine amine congener (XAC; 10(-6) M; Ado A(1), A(2A), and A(2B) receptor antagonist), or glibenclamide (Glib; 10(-5) M; K-ATP channel inhibitor) superfused over the preparation attenuated the local dilation (by 29.7 +/- 12.7, 61.8 +/- 9.0, and 51.9 +/- 14.9%, respectively), but only XAC and Glib attenuated the upstream dilation (by 68.9 +/- 6.8 and 89.1 +/- 6.4%, respectively). Furthermore, only Glib, when applied to the upstream site directly, attenuated the upstream dilation (48.1 +/- 9.1%). Neither XAC nor Glib applied directly to the arteriole between the local and the upstream sites had an effect on the magnitude of the upstream dilation. We conclude that NO, Ado receptors, and KATP channels are involved in the local dilation initiated by contracting muscle and that both KATP channels and Ado receptor stimulation, but not NO, play a role in the manifestation of the dilation at the upstream site.
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