Journal
ANNALS OF BOTANY
Volume 89, Issue 4, Pages 491-497Publisher
OXFORD UNIV PRESS
DOI: 10.1093/aob/mcf054
Keywords
protoplast fusion; genetic transformation; green fluorescent protein; GFP; woody plant; vital marker; Citrus reticulata; Citrus sinensis; Poncirus trifoliata; Agrobacterium tumefaciens
Categories
Ask authors/readers for more resources
Using a transgenic citrus plant expressing Green Fluorescent Protein (GFP) as a parent in somatic fusion experiments, we investigated the suitability of GFP as an in vivo marker to follow the processes of protoplast fusion, regeneration and selection of hybrid plants. A high level of GFP expression was detected in transgenic citrus protoplasts, hybrid callus, embryos and plants, It is demonstrated that GFP can be used for the continuous monitoring of the fusion process, localization of hybrid colonies and callus, and selection of somatic hybrid embryos and plants. (C) 2002 Annals of Botany Company.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available