Journal
MYCORRHIZA
Volume 12, Issue 2, Pages 61-66Publisher
SPRINGER
DOI: 10.1007/s00572-001-0148-3
Keywords
Andean alder; ectomycorrhiza; characterization and identification; PCR/RFLP
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Field ectomycorrhizae of Naucoria escharoides on Alnus acuminata (andean alder, aliso del cerro) are described in detail for the first time. Naturally occurring ectomycorrhizal roots were sampled beneath sporocarps of N. escharoides. The samples were taken from four natural forest plots at two homogeneous A. acuminata sites (Tucuman and Catamarca Provinces, Argentina). The ectomycorrhizae were characterized morphologically and compared by means of PCR/RFLP analysis of the internal transcribed spacer region of the nuclear rDNA. The most important morphological features of the ectomycorrhizae are a white to pale yellow mantle, simple to monopodial branches, hyaline emanating hyphae, abundant hyphal bundles emerging more or less perpendicularly from a plectenchymatous mantle, and an acute or rounded apex with or without a mantle. N. escharoides fruitbodies have white basal mycelium with emanating hyphae similar to those of andean alder ectomycorrhizae. The RFLP profiles of sporocarps and mycorrhizae were the same.
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