Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 68, Issue 4, Pages 2061-2065Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.68.4.2061-2065.2002
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A multiplex PCR was developed to simultaneously detect Naegleria fowleri and other Naegleria species in the environment. Multiplex PCR was also capable of identifying N. fowleri isolates with internal transcribed spacers of different sizes. In addition, restriction fragment length polymorphism analysis of the PCR product distinguished the main thermophilic Naegleria species from the sampling sites.
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