Protein kinase C activation by iridal type triterpenoids

Eleven iridal type triterpenoids from Iris tectorum and Belamcanda chinensis were examined for protein kinase C (PKC) activation and binding activity to PKC. Among the tested compounds, nine iridals showed dose-dependent activities, and a mutual relation between the two activities was also observed. 28-Deacetylbelamcandal, which has been found to be a new class 12-O-tetradecanoylphorbol 13-acetate type tumor promoter, showed the most potent activity in both tests. The structural requirements of the iridals inducing these activities were as follows: 1) a hydrophobic side-chain, 2) an E-methylidene aldehyde group at the C-1 position, and 3) a hydroxyl group at the C-26 position.