Journal
JOURNAL OF CELL BIOLOGY
Volume 157, Issue 1, Pages 79-89Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200112098
Keywords
vacuole fusion; Vam7p; SNAP-23; SNARE complex; PX domain
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Funding
- NIGMS NIH HHS [GM23377, R01 GM023377] Funding Source: Medline
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Vacuole fusion requires a coordinated cascade of priming, docking, and fusion. SNARE proteins have been implicated in the fusion itself, although their precise role in the cascade remains unclear. We now report that the vacuolar SNAP-23 homologue Vam7p is a mobile element of the SNARE complex, which moves from an initial association with the cis-SNARE complex via a soluble intermediate to the docking site. Soluble Vam7p is specifically recruited to vacuoles and can rescue a fusion reaction poisoned with antibodies to Vam7p. Both the recombinant Vam7p PX domain and a FYVE domain construct of human Hrs block the recruitment of Vam7p and vacuole fusion, demonstrating that phosphatidylinosital 3-phosphate is a primary receptor of Vam7p on vacuoles. We propose that the Vam7p cycle is linked to the availability of a lipid domain on yeast vacuoles, which is essential for coordinating the fusion reaction prior to and beyond docking.
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