Effect of pH on the interfacial tension of bilayer lipid membrane formed from phosphatidylcholine or phosphatidylserine

The effect of pH of an electrolyte solution on the interfacial tension of lipid membrane formed from phosphatidylcholine (PC) or phosphatidylserine (PS) was studied. The relationships were well described by an equation presented earlier based on the Gibbs isotherm but only in the proximity of the isoelectric point. Therefore, in this work models have been derived to describe the adsorption of the H+ and OH- ions at lipid surfaces formed from PC or PS, which would reproduce changes in interfacial tension more correctly, particularly in the ranges distant from the isoelectric point. In one model, the surface is continuous with uniformly distributed functional groups constituting the centres of HI and OH- ion adsorption while in the other the surface is built of lipid molecules, free or with attached H+ and OH- ions. In both models, the contributions of the individual lipid molecule forms to the interfacial tension of the bilayer were assumed to be additive. (C) 2002 Elsevier Science B.V. All rights reserved.