Journal
SCIENCE
Volume 296, Issue 5567, Pages 550-553Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1068999
Keywords
-
Categories
Ask authors/readers for more resources
Mammalian genetic approaches to study gene function have been hampered by the lack of toots to generate stable loss-of-function phenotypes efficiently. We report here a new vector system, named pSUPER, which directs the synthesis of small interfering RNAs (siRNAs) in mammalian cells. We show that siRNA expression mediated by this vector causes efficient and specific down-regulation of gene expression, resulting in functional inactivation of the targeted genes. Stable expression of siRNAs using this vector mediates persistent suppression of gene expression, allowing the analysis of loss-of-function phenotypes that develop over longer periods of time. Therefore, the pSUPER vector constitutes a new and powerful system to analyze gene function in a variety of mammalian cell types.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available