Phylogeny of the fungus Spilocaea oleagina, the causal agent of peacock leaf spot in olive

The fungus Spilocaea olcagina causes peacock leaf spot in olive. Virtually nothing is known about S, olcagina despite the loss of crop yield caused by this fungus. In order to get insight, an in vitro culture of the fungus has been established and its identity confirmed by amplified fragment length polymorphism analysis. Using this in vitro culture, we have cloned and analysed the DNA sequences of the 18S and 28S ribosomal RNA genes (rDNA) as well as the internal transcribed spacers (ITS) and 5.8S rDNA region of S. oleagina. Sequence analysis and comparison to other fungi determined that this fungus belongs to the Dothideomycetes class. We have also determined that S. oleagina is an anamorphic phase of a yet unidentified Venturia species based on phylogenetic analysis using the 28S rDNA and ITS sequences. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V, All rights reserved.