Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 955, Issue 1, Pages 125-131Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(02)00230-3
Keywords
derivatization; electrophoresis; enantiomer separation; triazines; amino acids; dichlorotriazinylamino-di-methylaminomethylphenazine
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An approach to the chiral separation of racemic mixtures of amino acids by means of micellar electrokinetic chromatography after derivatization with a new triazine spectroscopic reagent, 3-(4,6-dichloro-1,3,5-triazinylamino)-7-dimethylamino-2-methylphenazine (DTDP), has been evaluated. It was found that the derivatives of the aliphatic amino acids such as serine, valine and arginine, could produce a strong UV absorption at 282 nm, whose apparent molar absorptivities are of 10(4) M-1 cm(-1), and thus the concentration of the amino acids down to 3 X 10(-7) M can still give a detectable signal (SIN = 3). beta-Cyclodextrin (beta-CD) added to the buffer system was used as a chiral selector, and separation conditions were optimized. The presence of an organic modifier (2-propanol) was also a prerequisite for the chiral separation. The best results for the chiral separation of DTDP-amino acids were achieved in a mixed sodium dodecylsulfate-beta-CD-borate-2-propanol medium at pH 9.0. Compared to some of the commonly used derivatization methods, the present one offers a relatively stable derivative and strong U-V absorption for the spectroscopically inert amino acids, thus enabling amino acids to be separated and detected by CE even with a simpler UV detector. (C) 2002 Elsevier Science B.V. All rights reserved.
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