Journal
NATURE CELL BIOLOGY
Volume 4, Issue 5, Pages 343-350Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb783
Keywords
-
Categories
Funding
- NCI NIH HHS [CA76092, CA67771] Funding Source: Medline
- NCRR NIH HHS [M01RR00096] Funding Source: Medline
- NIAID NIH HHS [AI36224] Funding Source: Medline
- NIGMS NIH HHS [GM55279] Funding Source: Medline
Ask authors/readers for more resources
Current models evoke the plasma membrane ( PM) as the exclusive platform from which Ras regulates signalling. We developed a fluorescent probe that reports where and when Ras is activated in living cells. We show that oncogenic H-Ras and N-Ras engage Raf-1 on the Golgi and that endogenous Ras and unpalmitoylated H-Ras are activated in response to mitogens on the Golgi and endoplasmic reticulum ( ER), respectively. We also demonstrate that H-Ras that is restricted to the ER can activate the Erk pathway and transform fibroblasts, and that Ras localized on different membrane compartments differentially engages various signalling pathways. Thus, Ras signalling is not limited to the PM, but also proceeds on the endomembrane.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available