Journal
IMMUNOLOGY LETTERS
Volume 81, Issue 3, Pages 199-203Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0165-2478(01)00347-9
Keywords
lymphocyte subpopulations; reference ranges; flow cytometry
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Fluorescent monoclonal antibody labelling followed by a lysed whole blood method and flow cytometry was used to determine the lymphocyte subpopulations in 127 (64 males and 63 females) normal healthy individuals in the adult (age 18-59 years) Kuwaiti population. Relative percentages and absolute values of CD2+, CD3+, CD19+, CD4+, CD8+, HLADR+, CD56+, CD45RO+, and CD45RA+ cells were determined. The reference ranges were CD2+, 73-92% (0.95-2.99 x 10(9) per 1), CD3+, 64-85% (0.83-2.71 x 10(9) per l),CD19+, 6-22% (0.05-0.61 x 10(9) per l), CD4+, 34-54% (0.45 -1.65 x 10(9) per l), CD8+, 20-42% (0.29-1.17 x 10(9) per l), HLADR+, 4-23% (0.02-0.62 x 10(9) per l), CD56+, 4-22% (0.06-0.58 x 10(9) per l), CD45RO+, 16-53% (0.26-1.42 x 10(9) per l) and CD45RA+, 35-72% (0.34-2.05 x 10(9) per l). The mean CD4/CD8 ratio was 1.50+/-0.35. CD3+ cells were positively correlated to both CD4+ and CD8+ cells (P < 0.001), and CD4+ cells showed a significant positive correlation with CD8+ cells (P < 0.001). (C) 2002 Elsevier Science B.V. All rights reserved.
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