Journal
DEVELOPMENTAL CELL
Volume 2, Issue 5, Pages 593-605Publisher
CELL PRESS
DOI: 10.1016/S1534-5807(02)00168-5
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Funding
- NCI NIH HHS [CA 58689] Funding Source: Medline
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Production of the essential phospholipid PI4P at the Golgi by the Pik1 kinase is required for protein secretion, while a distinct pool of PI4P generated by the Stt4 kinase is critical for normal actin cytoskeleton organization. We identify a transmembrane protein that stabilizes Stt4 at the plasma membrane where it directs synthesis of PI4P, which is required for activation of the Rho1/Pkc1-mediated MAP kinase cascade. Inactivation of Stt4 or the PI4P 5-kinase Mss4 results in mislocalization of the Rho-GTPase GEF Rom2. Rom2 binds PI4,5P(2) through its PH domain and represents the first identified effector in the Stt4-Mss4 pathway. Based on these results, we propose that Stt4-Mss4 generates PI4,5P2 at the plasma membrane, required to recruit/activate effector proteins such as Rom2.
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