Journal
LUMINESCENCE
Volume 17, Issue 3, Pages 141-149Publisher
WILEY
DOI: 10.1002/bio.681
Keywords
lysophospholipids; luminol-amplified chemiluminescence; MALDI-TOF mass spectrometry; NADPH oxidase
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In this study, the effects of exogenous lysophospliolipids-lysophosphatidic acid. lysophosphatidyleholine. lysophosphatidyl ethanol amine and lysophosphatidyl serine-on the kinetics of reactive oxygen species (ROS) production by human neutrophils are described. The ROS production by human neutrophils was monitored by luminol-amplified chemiluminescence after cell stimulation with the chemotactic tripeptide, fMLP, or with the phorbot ester, PMA. The interaction of lysophospholipids with the membrane of human neutrophils was additionally tested by mass spectrometry. Lysophosphatidylcholine showed the most pronounced effect on the chemiluminescence pattern, as well as the intensity of the fMLP and PMA-stimulated cells. whereas lysophosphatidic acid showed a slight priming effect when fMLP was used for stimulation. In the case of fMLP-stimulated cells, lysophosphatidyleholine inhibited the first phase and enhanced the second phase of chemiluminescence, whereas the chemiluminescence of PMA-stimulated neutrophils was inhibited in a concentration-dependent manner. We conclude that lysophosphatidylcholine is able to interact with protein kinase C-dependent signalling pathways leading to NADPH oxidase activation. Copyright (C) 2002 John Wiley Sons, Ltd.
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