Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 66, Issue 5, Pages 1174-1175Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.66.1174
Keywords
pyridylamination; oligosaccharide; fluorescence; glycome
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We developed a convenient method for purification of PA-oligosaccharides to remove contaminants originating from natural fluorescent materials, and excess reagents as well as by-products of tagging reactions in glycan analysis. The method, using a C18-cartridge, is simple and powerful to remove them. Several examples of experiments that showed the usefulness of this purification method are described in this report.
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