Journal
MOLECULAR BIOTECHNOLOGY
Volume 21, Issue 1, Pages 43-50Publisher
HUMANA PRESS INC
DOI: 10.1385/MB:21:1:043
Keywords
Coffea arabica; somatic embryogenesis; gene expression; differential display
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Molecular and biochemical studies of somatic embryogenesis may help to shed light on the mechanisms governing this phenomenon. In this article, a differential display analysis approach was employed to investigate the changes taking place during the induction of somatic embryogenesis in leaf explants and suspension cultures of coffee. Cloned fragments show homologies to several proteins reported in databases, but only one has previously been described as regulated during somatic embryogenesis. By a reverse dot blot modification, the expression pattern of such fragments was evaluated.
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